HPLC Training

Our liquid chromatography training program covers LC theory, instrument operation, method setup, routine maintenance, troubleshooting, and data analysis. Training can be tailored for entry-level or experienced users and delivered onsite or virtually.

Entry-level trainees can learn the fundamentals of liquid chromatography before progressing to instrument operation and data analysis. Experienced users can build on their current knowledge with greater emphasis on method optimization, maintenance, and troubleshooting.

liquid chromatography training covers fundamentals, operation, and more.

Topic Areas

Liquid Chromatography Theory and System Overview

Learn the principles of liquid chromatography and the roles of the solvent delivery system, autosampler, column compartment, detector, and data system.

Mobile Phases and Solvent Management

Understand solvent selection, preparation, filtration, degassing, and the differences between isocratic and gradient separations.

Columns and Separations

Learn column selection, installation, handling, storage, and the factors that affect retention, selectivity, efficiency, and peak shape.

Methods and Data Acquisition

Set up instrument methods and sequences, prepare the system for analysis, establish operating conditions, and run samples consistently.

Data Analysis and Interpretation

Understand chromatograms, retention times, integration, calibration, peak identification, quantitative results, and reporting.

Routine Maintenance

Learn routine maintenance for pumps, autosamplers, detectors, solvent lines, and other system components to support reliable operation.

Troubleshooting

Identify and troubleshoot common problems including pressure changes, leaks, carryover, baseline noise or drift, retention-time shifts, and poor peak shape.

 

More Information

Our liquid chromatography training program helps users understand how each LC component contributes to the separation and the quality of the resulting data. Trainees learn how to prepare mobile phases, select and care for columns, configure operating conditions, create methods and sequences, and process analytical results.

The course also covers routine maintenance and practical troubleshooting techniques. Users learn how to recognize common symptoms, isolate potential causes, and take appropriate corrective action when chromatography or instrument performance changes.

Course content can be customized around your laboratory’s instruments, software, applications, and the experience level of your team.

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Other Courses

ICP-MS Training

Learn ICP-MS fundamentals, routine operation, method setup, interference management, maintenance, troubleshooting, and data analysis.

LC/MS Training

Learn LC/MS operation, maintenance, troubleshooting, data acquisition, and qualitative and quantitative data analysis.